Therapeutic effect of the transplantation of trans-TrkC gene neural stem cells on spinal cord injury
- VernacularTitle:转TrkC基因神经干细胞移植对脊髓损伤的治疗作用
- Author:
Ri-Sheng LIANG
;
Liang-Fu ZHOU
;
Rong ZHANG
;
Ying MAO
;
Wei-Zhong YANG
;
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries;
Stem cells;
Cell transplantation;
Tyrosine Kinase C
- From:
Chinese Journal of Trauma
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of trans-TrkC gene neural stem cells (NSCs)on the recovery of neural function after spinal cord injury.Methods Sixty SD rats were ran- domly divided into six groups:normal control group(A),hemisection group(B),NSCs transplantation grnup(C),NSCs transplantation with the regional application of NT-3 group(D),trans-TrkC gene NSCs transplantation group(E)and trans-TrkC genc NSCs transplantation with the regional application of NT- 3 group(F),10 rats in each group.Nine days after the set up of animal models,cell transplantation into the injured spinal cord was performed.The BBB locomotor score was calculated,and MEP(motor evoked potential)and SEP(somatesensory evoked potential)were pedormed two months after cell transplanta- tion.Results Two months after cell transplantation,the BBB locomotor score was partly recovered, and the MEP and SEP(somatosensory evoked potential)results were also markedly improved in Group F, which indicated the restoration of the upward and downward nerve conduction function of the injured spinal cord.But it seemed that the restoration of the downward nerve conduction was better than that of the up- want,and the extent of the improvement of MEP and SEP results was larger than that of motion function recovery.The onset latency,peak to peak amplitude of MEP and SEP,and the BBB score of Group F re- stored the best compared with the other groups,and the differences were statistically significant(P
