Effects of 1,25(OH)_2D_3 on cell proliferation in mouse osteoblasts
- VernacularTitle:1，25-二羟基维生素D_3对小鼠成骨细胞增殖的影响
- Author:
Qing-Xian TIAN
;
Gong-Yi HUANG
;
- Publication Type:Journal Article
- Keywords:
caleifediol;
Osteoblasts;
Cell cycle
- From:
Chinese Journal of Geriatrics
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 1,25(OH)_2D_3 on cell proliferation and cell cycle progression in mouse osteoblasts.Methods Sterile bones of skull of mouse were taken from 30 newborn mouse,and the osteoblast were separated by enzyme digestion methods.After 1,25(OH)_2D_3 in different concentrations were added into culture medium,the effects of 1,25(OH)_2D_3 on cell proliferation of mouse osteoblasts and on cell cycle progression were examined by mono-nuclear celldirect cytotoxicity assay(MTT)reduction assay and flow cytometry respectively.Results After 24,48,72 h of 1,25(OH)_2D_3 incubation,the cell number of osteoblast had significant difference among groups of 1,25(OH)_2D_3 of 10~(-8),10~(-9),10~(-11)mol/L.Significant differences were found in the cell cycle progression in response to 1,25(OH)_2D_3 treatment from the Gl(84.30?1.90)to the G2-M (7.70?0.667)and S(8.00?1.42)phases when compared with those in the control group. Conclusions Cell proliferation of mouse osteoblasts can be inhibited by 1,25(OH)_2D_3 in a concentration-dependent manner.
