Expression and significance of major histocompatibility complex classⅡgene in multiple organ dysfunction syndrome
- VernacularTitle:主要组织相容性复合体Ⅱ类基因在多器官功能障碍综合征中的表达及意义
- Author:
Chen CHAI
;
Guo-En FANG
;
Xian SHEN
;
Fu-Lin LIN
;
Ming-Ming NIE
;
Tian-Hang LUO
;
Yang YUAN
;
Jian-wei BI
- Publication Type:Journal Article
- Keywords:
Major histocompatibility complex classⅡgene;
Multiple organ dysfunction syndrome;
Transactivator;
Cytokines
- From:
Chinese Journal of Trauma
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of major histocompatibility complex classⅡgene in multiple organ dysfunction syndrome.Methods Two-hit porcine model of MODS was duplicated in 18 swine that were randomly assigned into experimental group(Group M,n=9) and control group(Group C,n=9).The Group M was given compound factors including hemorrhagic shock,reperfusion injury and endotoxemia,and the Group C only underwent anesthesia and arterious/ve- nous eannula.After seven days,the animals were killed to remove splenic tissues fro extracting total RNA by Trizol method.The primer of SLA-DQA(MHC classⅡgene of swine)was designed to construct cD- NA by reverse transcription and the quantity of SLA-DQA mRNA detected with real time fluorescent quan- titative polymerase chain reaction(real time FQ-PCR).The standard curve was described by UVP com- puter image analysis system.Results The mortality of Group M was 78%(7/9),and the incidence rate of MODS was 89%(8/9).The expressing quantity of Group M was(1.376?1.006)?10~3,signifi- cantly lower than(5.330?3.053)?10~3 of Group C(P＜0.01).Conclusion Duplication of por- cine MODS model is satisfactory.Down-regulation of MHC classⅡgene may be due to control of classⅡtransactivator(CⅡTA)and release of multiple eytokine,such as TNF-?and IL-10.
