Ciliary neurotrophic factor-coated polyglecolic-polylactic acid nerve conduits to repair canine tibial nerve defects
- VernacularTitle:睫状神经营养因子涂层的聚羟基乙酸聚乳酸神经导管修复犬胫神经缺损
- Author:
Zhunli SHEN
;
Hua SHEN
;
Jing ZHANG
;
Peihua ZHANG
;
Wenzu WANG
;
Nanliang CHEN
;
Zhiqing TAN
;
Yongqing WANG
;
Feng LIAN
;
- Publication Type:Journal Article
- Keywords:
Peripheral nerve;
Nerve conduit;
Pulsed plasma;
Ciliary neurotrophic factor;
Canine
- From:
Chinese Journal of Microsurgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ciliary,neurotrophic factor (CNTF)-coated polyglycolic and polylactic acid (PGLA) nerve conduits treated by pulsed plasma to repair canine tibial nerve defects. Methods A 2.5 cm long tibial nerve defect was made in eighteen cross-bred dogs.The nerve defects were re- constructed by three different methods:group A:pulsed plasma treated and CNTF coated PGLA nerve conduits (n=6);group B:PGLA nerve conduits alone (n=6);group C:nerve autografts (n=6).HE staining, Massons' trichrome staining,S-100 immunostaining,electrophysiological test and axon counting were used to evaluate the results of nerve regeneration in three groups.In addition,the dynamic walking pattern was recor- ded individually.The observation period lasted for three months.Results All nerve conduits were well vas- cularized and mostly degraded as well as absorbed.It was found that the regenerating axons could traverse all nerve conduits.In regard to nerve conduction velocity and axon counting there was no significant difference be- tween group A and group C (P＞0.05),while the data of group A and group C were significantly better than those of group B(P＜0.05).The dogs in group A and C recovered nearly normal walk pattern while those in group B were still crippled.Conclusion Pulsed plasm-treated and then CNTF-coated PGLA nerve conduits could effectively repair 2.5-cm-long canine tibial nerve defects,and the effect is similar to that of autografts.
