Preventive effect of TGF-?_1 neutralizing antibody on flexor tendon adhesion from operation
- VernacularTitle:TGF-?_1多克隆抗体预防屈指肌腱术后粘连的实验研究
- Author:
Jin-Tang WANG
;
Yin-Gang ZHANG
;
Jun LI
;
Nai-Gui BI
;
Xue-Zhe HAN
;
Xiao-Wei ZHANG
;
- Publication Type:Journal Article
- Keywords:
Transforming growth factor(TGF);
Tendon;
Adhesion;
Microsurgery
- From:
Chinese Journal of Microsurgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effect of TGF-?_1 neutralizing antibody on flexor tendon adhesion from operation.Methods One hundred and eight leghon cocks performed anastomonsis op- eration were divieded into three groups randomly,as normal saline(control group),5?g/ml group,10?g/ml group of TGF-?_1,antibody.At 1 st,3rd,8th and 12th weeks respectively after operation,the flexor biomechan- ics test,HE staining,Masson staining,Sirius red-polarization staining and TGF-?_1 immunohistochemistry stai- ning were used.Results The max of strength of tendon and the stimulate active flexor from the experiment groups(5?g/ml group,10?g/ml) are higher than from the control group,The max of strength of tendon of the experiment groups are less at 8th weeks,and no difference at 12th weeks from the control group;Compared with the control group,the 10?g/ml group were less shorten the progress of inflammation and accelerated the progress of molding;In the experiment groups(5?g/ml group,10?g/ml),the density of the collagenⅠtype were less,the ratio ofⅠ/Ⅲcollagen and expression of the TGF-?_1 were decreasing.Condusion The study showed that applying of TGF-?_1 muhiclonal neutralizing antibody can inhibit efficiently the function of the TGF-?_1 during the flexor tendon repair,reduce tendon adhesion and scar fromation,however has no affec- tion of tendon intensity,suggesting it is a latent and efficient method for preventiong flexor tendon from adhe- ring after operation.
