Effect of Astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection
- VernacularTitle:落新妇甙诱导混合细胞培养中活化的T细胞凋亡及其机制
- Author:
Si-Hai GAO
;
Ping LI
;
Tao CHEN
;
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Astilbin;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection.Methods Cardiomyocytes of BALB/ c mouse and spleen cells of C57BL/6 mouse were separated.The cardiomyocytes(2?10~5/ml)as irri- tation cells and spleen cells(1?10~6/ml)as responsers were mixed and cultured.The model of mouse heart transplantation with acute rejection in vitro was established.Three groups were set up:control group,Astilbin(15/?g/ml) group,Astilbin+anti-CTLA-4 mAb 9H10 (30?g/ml)group.Apoptosis of T cells was observed by TUNEL.The expression of CTLA-4 in activated T cells was detected by RT-PCR and Western blot.Results Apoptosis indexes of activated T cells in Astilbin group were sig- nificantly higher than those in the control group(73.4%?12.5% vs 35.1%?9.2%,P＜0.01). The expression of CTLA-4 in Astilbin group was significantly higher than control group(P＜0.01), but there was no apparently difference between control group and Astilbin+CTLA-4 mAb group(P＞0.05).Conclusion Astilbin induces apoptosis of activated T cells in heart transplantation,which may be partially related to its enhanced expression of CTLA-4.
