Myocardial protective effects of“integrated strategy”with Na~+/H~+ exchange inhibitor (HOE-642) plus modified K/Mg cold blood cardioplegia on canine heart grafts preserved
- VernacularTitle:HOE-642联合改良K/Mg冷血心麻液对犬供心的保护作用
- Author:
Da-De HUANG
;
Weimin CHEN
;
Cheng-Hua ZHONG
;
Guangwen CHEN
;
Lixin XU
;
- Publication Type:Journal Article
- Keywords:
Organ preservation solutions;
Canine;
Heart transplantation
- From:
Chinese Journal of Organ Transplantation
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the myocardial protective effects of“integrated strategy”with HOE-642 plus modified K/Mg on canine heart grafts.Methods Sixteen donors-recipient pairs of adult canines were divided into the“integrated strategy”group,i.e.,donor hearts were protected with HOE-642 plus K/Mg(IS,n=8)and the routine method(RM,n=8)group.Both groups un- derwent hypothermia stored for 4 h,followed by orthotopic transplantation.Cardiac heamodynamics was measured and myocardial ultrastructure and myocardial water content were evaluated.Results One of 8 animal died of aortic anastomoses leakage in each group,and the rest could wean from car- diopulmonary bypass successfully.Preservation of left ventricular contractility,as well as left ventric- ular compliance was significantly better in the IS group than in the RM group(P＜0.05),also,per- centage of myocardial water content was significantly lower in the IS group than in the RM group(P＜0.05).Myocardial ultrastructures were not damaged significantly in both groups,however,better myocardial preservation were seen in the IS group.Conclusion Both the clinically used“routine meth- od”and“integrated strategy”might provide safe donor heart preservation for 4 h,but the later scheme conferred more optimal effects with alleviating the myocardial swelling,preserving myocardial ultra- structure integrity and enhancing postischemic-reperfusion cardiac functional recovery.
