The expression of endogenous TGF-?1 of rabbit pericranium during osteogenesis induced by rhBMP-2
- VernacularTitle:骨形态发生蛋白-2在诱导兔颅骨再生过程中对转化因子-?1表达的影响
- Author:
Yan-Kai XU
;
Ying-Ming YANG
;
Chu-Wei CAI
;
Wei-Qiang CHEN
;
Qi-Hui ZHANG
;
Wangan LI
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Bone regeneration;
Transforming growth factor beta
- From:
Chinese Journal of Trauma
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of exogenous rhBMP-2(recombinant human bone morpho- genetic protein-2)on the expression of endogenous TGF-?1 during osteogenesis in rabbits.Methods After successfully duplicating experimental rabbit skull defect model,we detected and analyzed the expression of en- dogenous TGF-?1 of the pericranium in different stages by using immunohistochemieal method in both the ex- perimental group and the control group.Results In the experimental group a lot of mesenchymal cells with positive expression of endogenous TGF-?1 of the pericranium aggregated in duramater in the early stage after operation,and the expression level increased rapidly with the differentiation of mesenchymal cells,and reached the peak at 17,19 day postoperation.Positive cell number of TGF-?1 of pericranium was statistically analyzed in both groups at 7,15,21 days,showing significant difference(t-test,P＜0.01 ).Conclusion Exogenous rhBMP-2 can induce and enhance the expression of endogenous TGF-?1 during osteogenesis.
