The relation between the spatial cognitive deficit in MWM and the changes of cholinergic neuron after brain concussion in rats
- VernacularTitle:脑震荡鼠水迷宫空间认知障碍与脑胆碱能神经元变化的关系
- Author:
Hai-Ying LIN
;
Chun-Yun WU
;
Jian-Yun YU
;
Ze-Yun GUO
;
Bing-Ying XU
;
Xiao-Lin ZHAO
;
Xu-Dong ZHAO
- Publication Type:Journal Article
- Keywords:
Brain concussion;
Cognition;
Cholinergic neuron;
Rats
- From:
Chinese Journal of Trauma
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between cognitive dysfunction and cholinergic neuron changes in basal forebrain(BFB)and brainstem reticular formation(BSRF)areas after brain con- cussion(BC)in rats.Methods Eighty-four Spragne-Dawley rats weighing 250-310g were used.The BC rat models were reproduced by a self-made simple pendulum impact device,then the rats were ran- domized into one control group and six experimental groups(1 day,2 day,4 day,8 day,16 day,and 24 day groups;n=12 in each group).A Morris Water Maze(MWM)test was used to assess learning and memory function of the rats.Cholinergic neurons in the BFB and BSRF were identified with choline acetyltransferase(CHAT)antibody and quantitated.Results Compared with the control group,the la- tency to find the platform in MWM was much longer on days 1-3 after BC,but there was no statistical difference on days 4-21 after BC.The cell number and the ChAT expression activity of cholinergic neu- rons in the BFB were obviously decreased after BC,and reached the lowest level at 8 days after BC,then increased gradually and nearly reached the normal level at 24 days.The ChAT expression activity in BSRF declined on the first 2 days after BC,then went up gradually,and reached the peak at the 24th day.Conclusion The spatial cognition deficit of BC rats can be detected by MWM in the early period (1-3 days after BC).There are significant changes in the number and ChAT expression activity in BFB and BSRF after BC.The change of cholinergic neurons may be correlated with cognitive deficits in BC rats.
