Repair of canine segmental mandibular defects by using autogenous bone marrow stromal cells and?-tricalcium phosphate
- VernacularTitle:自体骨髓间充质干细胞复合?-磷酸三钙修复犬下颌骨节段缺损
- Author:
Jie YUAN
;
Lian ZHU
;
Guang-Peng LIU
;
Feng XU
;
Yu-Lai WONG
;
Lei CUI
;
Wei LIU
;
Yi-lin CAO
- Publication Type:Journal Article
- Keywords:
Mandibular injuries;
Bone marrow stromal cells;
?-tricalcium phosphate;
Tissue engineering
- From:
Chinese Journal of Trauma
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To repair segmental mandibular defects with autogenous bone marrow stromal cells(BMSCs)and?-triealcium phosphate.Methods Isolated BMSCs were in vitro expand- ed.A 3 cm-long segmental mandibular defect was created at right mandible in 12 canines,of which de- fects in six canines were repaired with BMSCs and?-tricalcium phosphate(?-TCP)and that in other six cases repaired with?-TCP,which was used as control.The engineered bone was evaluated by X-ray, CT,DXA,gross and histological examination,immunohistochemistry and biomechanical test 4,12,26,32 weeks after operation respectively.Results In induced BMSCs,histochemistry showed AKP activity. Oral X-ray showed obvious callus formation 4-26 weeks after operation in experimental group but minimal bone formation in control group.At 32 weeks after operation,gross observation,X-ray and CT demonstra- ted well bony-union in experimental group but bony-nonunion in control group.DXA indicated that the bone density of experimental group was significantly higher than that of control group.Biomechanical test revealed no statistical difference upon mechanical strength of mandibula between experimental group and normal group.Conclusions Canine segmental mandibular defects can be well repaired with the tissue- engineered bone generated by autogenous osteogenic BMSCs and?-TCP scaffold.
