Role of apoptosis of neutrophils and vascular endothelial cells and changes of relative factors in the pathogenesis of traumatic sepsis at the advanced stage
- VernacularTitle:中性粒细胞和内皮细胞凋亡及相关因子改变在晚期创伤性脓毒症发病中的作用
- Author:
Yu-Miao WEI
;
Yu-Hua LIAO
;
Shu-Juan CHU
;
Quan-Jun ZHOU
;
Guo-Rong WANG
;
Chao-Hui WANG
;
Long-Xian CHENG
- Publication Type:Journal Article
- Keywords:
Sepsis;
Neutrophil;
Endothelial cells;
Apoptosis
- From:
Chinese Journal of Trauma
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of apoptosis of neutrophils and vascular endothelial cells and changes of relative cytokines and thrombotic factors in the pathogenesis of traumatic sepsis at the advanced stage.Methods The venous blood was collected from the patients with traumatic sepsis at advanced stage and traumatic patients without sepsis and healthy subjects.The peripheral hlood mononu- clear cells(PBMC)and neutrophils were isolated and cultured.The apoptosis of neutrophils and vascular endothelial cells was assayed,and the level of IL-4,IL-10 in PBMC culture supernatants were deter- mined,and the tissue factor(TF)and vW factor(vWF)of peripheral plasma were measured.Results The percentages of apoptosis of neutrophils and numbers of apoptotic circulating vascular endothelial cells were higher than that of traumatic patients without sepsis and healthy controls significantly.And the levels of anti-inflammatory cytokines IL-4,IL-10 and thrombotic factors TF,vWF in traumatic sepsis were elevated than that of traumatic patients without sepsis and controls too.Conclusion Immune suppres- sion and abnormal thrombotic state may be one characteristic of traumatic sepsis at advanced stage,which perhaps involveds in the pathogenesis of traumatic sepsis at the advanced stage and multiple organ dys- function syndrome.
