Culture of rat skeletal muscle satellite cells and their response to stimulation by insulin-like growth factor
- VernacularTitle:肌卫星细胞体外培养及其对胰岛素样生长因子Ⅰ刺激的反应
- Author:
Gang LI
;
Qiang LIU
;
- Publication Type:Journal Article
- Keywords:
Satellite cells;
Insulin-like growth factor;
Cell cultivation;
Muscular atrophy
- From:
Chinese Journal of Trauma
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the proliferation and differentiation properties of rat skeletal muscle satellite cells that are dissected,cultured and purified in vitro and investigate their response to stimulation by insulin-like growth factor I(IGF-I).Methods The cells were isolated from Wistar rats by two-step enzyme digestion and purified by the Percoll density centrifugation to observe their prolif- eration and differentiation properties and draw growth curve and fusion rate curve.Then,the response of the satellite cells to stimulation by IGF-I at various concentrations was determined by using MTT meth- od.Results Plenty and purified satellite cells were obtained via these methods.Conclusion IGF- I at appropriate concentration(100 ng/ml)can promote the proliferation and differentiation of rat skele- tal muscle satellite cells.
