Relationship between CIDE-B change and neuronal apoptosis under various injuring conditions
- VernacularTitle:不同损伤条件下CIDE-B的变化与神经元凋亡的关系
- Author:
Hong-Zhen SUN
;
Zheng-Guo WANG
;
Pei-Fang ZHU
;
- Publication Type:Journal Article
- Keywords:
Peripheral nerves;
Wounds,gunshot;
Apoptosis;
CIDE-B gene
- From:
Chinese Journal of Trauma
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between CIDE-B change and apoptosis by detecting expression of CIDE-B gene and protein under various injuring conditions(firearm injury and in- cision injury)and comparing neuronal apoptosis.Methods A total of 108 New Zealand rabbits were randomized into firearm injury group,incision injury group and control group.Samples were harvested at different time points after injury for measuring CIDE-B mRNA expression level by RT-PCR,demonstrating morphological distribution of CIDE-B mRNA by in situ hybridization and determining CIDE-B protein level by Western blot.Results Following nerve injury,CIDE-B mRNA expression in spinal cord was en- hanced markedly in the firearm injury group,increased from at day 1,reached peak at day 7,lasted for two weeks and declined after four weeks.CIDE-B mRNA expression was increased late,with small range in the incision injury group.The changes in CIDE-B protein level accorded with those in CIDE-B mRNA level.There was slight CIDE-B mRNA expression,distributing in spinal cord neuronal cytoplasma,in spinal cord tissues in the control group.Conclusions Following peripheral nerve injury,expressions of CIDE-B mRNA and protein are enhanced in spinal cord neurons and distributed in the grey matter of spi- nal cord.Such enhancement is more marked after firearm injury than incision injury.
