Evaluation of a competitive ELISA for antibody detection against avian influenza virus.
10.4142/jvs.2009.10.4.323
- Author:
Dae Sub SONG
1
;
Youn Jeong LEE
;
Ok Mi JEONG
;
Yong Joo KIM
;
Chan Hee PARK
;
Jung Eun YOO
;
Woo Jin JEON
;
Jun Hun KWON
;
Gun Woo HA
;
Bo Kyu KANG
;
Chul Seung LEE
;
Hye Kwon KIM
;
Byeong Yeal JUNG
;
Jae Hong KIM
;
Jin Sik OH
Author Information
1. Green Cross Veterinary Products, Youngin 446-569, Korea.
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
avian influenza virus;
competitive ELISA;
surveillance
- MeSH:
Animals;
Antibodies, Viral/*blood;
Birds;
Enzyme-Linked Immunosorbent Assay/methods/*veterinary;
Horses;
Influenza A virus/*immunology;
Influenza Vaccines/immunology;
Influenza in Birds/blood/*immunology/prevention & control;
Sensitivity and Specificity;
Serologic Tests;
Species Specificity;
Swine
- From:Journal of Veterinary Science
2009;10(4):323-329
- CountryRepublic of Korea
- Language:English
-
Abstract:
Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or following vaccination of the AIV H9 or H5 subtypes in chickens, ducks and geese. Next, we tested the sensitivity and specificity of the cELISA with sera from twelve different animal species, and compared these results with those obtained by the hemagglutination-inhibition (HI) test, the "gold standard" in AIV sera surveillance, a second commercially-available cELISA (IZS ELISA), or the agar gel precipitation (AGP) test. Compared with the HI test, the sensitivities and specificities of cELISA were 95% and 96% in chicken, 86% and 88% in duck, 97% and 100% in turkey, 100% and 87% in goose, and 91% and 97% in swine, respectively. The sensitivities and specificities of the cELISA in this study were higher than those of IZS ELISA for the duck, turkey, goose, and grey partridge sera samples. The results of AGP test against duck and turkey sera also showed significant correlation with the results of cELISA (R-value >0.9). In terms of flock sensitivity, the cELISA correlated better with the HI test than with commercially-available indirect ELISAs, with 100% flock sensitivity.
