Reconstruction of rabbit knee joint cartilage defect using tissues engineering method
- VernacularTitle:组织工程化软骨修复兔膝关节软骨缺损的实验研究
- Author:
Hongfeng JIANG
;
Wei WEI
;
Yun-Chuan XIE
;
Rong-Rui LI
;
Rao-Sheng ZHAI
;
Ri-Guang WANG
;
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Articular cartilage;
Atelocollagen;
Rabbit
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estimate curative effect of reconstruction of rabbit knee joint cartilage defect with the homogeneitic tissue engineered cartilages.Methods The chondrocytes were isolated and collected from articular cartilages of eight New Zealand white rabbits.The tissue engineered cartilages after culturing chondrocytes and atelocollogen for two days.Cartilage defects were created in both keen joint of twenty-six rab- bits.Complexes of chodrocytes and atelocollagen was grafted into the defect of left knee joint at once as experi- mental group,and no implantation were served as control.General and histological examination were respec- tively performed in both group at four weeks and eight weeks after surgery.Results After implantation,the defects were filled with cartilaginous tissue in experiment group,while there were only tissue in control group. Histologically,defective areas were filled with chondrocytes in experiment group,but only fibroblast in control group.Conclusion The implantation of the tissue engineered cartilages contenting with chondrocytes and atelocollogen can effectively improve reconstruction of rabbit knee joint.
