Effect of Ti-6AL-4V particles on morphology and function of osteoclasts:an in vitro study
- VernacularTitle:钛-6铝-4钒颗粒对破骨细胞形态和功能的影响
- Author:
Yuan-Qing MAO
;
Zhen-An ZHU
;
Sheng-Li XIA
;
Hong-Bin LI
;
Xiao-Qing WANG
;
Ting-Ting TANG
;
Qing-Wei JIA
- Publication Type:Journal Article
- Keywords:
Osteoclasts;
Bone resorption;
Prosthesis failure;
Ti-6AL-4V
- From:
Chinese Journal of Trauma
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ti-6AL-4V particles on morphological and func- tional changes of osteoclast in vitro.Methods Mature osteoclasts separated from New Zealand Rabbits were cultured on glass slices and cortical bone slices.The experimental group was stimulated by Ti-6AL- 4V particles at concentration of 0.1 mg/ml.The cells were stained with TRAP at different culture time to observe the morphological variety.The bone resorption pits on bone slices were stained by toluidine blue and the resorption areas analyzed by computer image analysis software.Results Osteoclasts phagocy- tosed the particles,with irregular shapes,deeper TRAP stain and earlier apoptosis.Stimulation by Ti- 6AL-4V particles brought about larger area of bone absorption lacuna.Conclusion Osteoclasts have the ability to phagocytose Ti-6AL-4V particles,which leads to morphological and functional changes and enhances bone resorption.
