Development and application of the method for the detection of human IL-6 mRNA
- VernacularTitle:RT-qPCR检测人IL-6 mRNA的方法建立与运用
- Author:
Li-Lin ZOU
;
Jian-Xin LV
;
- Publication Type:Journal Article
- Keywords:
Interleukin 6;
RNA, messenger;
Reverse transcription-polymerase chain reaction;
Real-time;
Quantification
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop and apply a method for real-time quantifying IL-6 mRNA.Methods:By Ficoll-Hypaque density gradient centrifugation, human peripheral blood mononuclear cells(PBMC) were isolated from whole blood treated with ethylenediaminetetraacetic acid(EDTA). Total RNA extracted from the PBMC in trizol solution was reverse transcribed to cDNA, which used as templates for fluorecent real-time quantitative PCR amplification of IL-6. PCR system consists of IL-6 primer, SBY Green Ⅰ and so on.Results:The method is wide linear range, sensitive, specific, reproducible and simple.Conclusion:The method can accurately quantify IL-6 mRNA.
