Alterations in KCNJ4 gene expression in human temporal lobe epilepsy
- VernacularTitle:难治性颞叶癫癇患者脑内KCNJ4基因表达的研究
- Author:
Xun-Yi WU
;
Zhen HONG
;
Xiang GAO
;
Guo-Xing ZHU
;
Chuan-Zhen LV
;
- Publication Type:Journal Article
- Keywords:
Epilepsy, temporal lobe;
Potassium channels, inwardly-rectifying;
Gene expression
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the possible molecular pathogenesis of intractable temporal lobe epilepsy. The potassium ion channel gene KCNJ4 encodes one of the subfamilies of Kir channels, Kir2.3 subunit, which may play an important role in modulating neuronal excitation. Interference in the function or expression of this gene would cause disturbance of ionic concentrations, thus leading to seizure activity. Methods Reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis were used to measure the expression alterations of KCNJ4 mRNA as well as its protein product Kir2.3 channel in temporal cortex samples from patients who had undergone temporal lobectomy for intractable epilepsy (n=12). Tissue from 10 subjects who did not have epilepsy served as controls. Results The expression of KCNJ4 mRNA (0.438?0.178) and its protein Kir2.3 (M 50=0.063) were significantly decreased in epileptic brain compared with the controls (P
