The effect of Htr- antisense oligodeoxynucleotide in combination with DDP on human gastric carcinoma transplanted subcutaneously in nude mice in vivo
- VernacularTitle:hTR反义PS-ODN联合顺铂对人胃癌裸鼠皮下移植瘤作用的体内研究
- Author:
Xuewen WU
;
Bangmao WANG
;
Xiaocang CAO
;
- Publication Type:Journal Article
- Keywords:
Antisense Oligodeoxynucleotide;
Gastric cancer cell line;
Tumor transplanted subcutaneously in nude mice;
Relative telomerase activity
- From:
Cancer Research and Clinic
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether hTR antisense PS ODN has anticancer effect and the effect of increasing the susceptibility of tumor to DDP, we explore the effect of hTR antisense oligodeoxynucleotide in combination with DDP on human gastric carcinoma transplanted subcutaneously in nude mice in vivo. Methods The model of human gastric carcinoma transplanted subcutaneously was established in thirty nude mice, then divided randomly into 5 groups (Control group, ASODN group, RODN group, DDP group and ASODN+DDP group). The weight of nude mice was measured, and the tumor growth inhibitory rate was calculated. The relative telomerase activity was quantitatively measured by TRAP PCR ELISA methods. Results The maximum tumor inhibitory rate in ASODN+DDP group, ASODN group, DDP group and RODN group was 94.2 %, 84.3 %, 92.8 % and 26.9 % respectively. There was significant difference in the relative telomerase activity among four treatment groups. Conclusions The results suggested that hTR antisense PS ODN may act as a specific tumor growth inhibitor and telomerase activity inhibitor may be in sequence specific manner, and have the effect of increasing the susceptibility of transplanted tumor to DDP.
