The study on the features of dopamine secretion in microencapsulated cells of bovine retinal pigmentary epithelial cells in vitro
- VernacularTitle:藻酸钠-多聚赖氨酸-藻酸钠微囊化牛视网膜色素上皮细胞多巴胺分泌特性的研究
- Author:
Wang-Chun DU
;
Jian WANG
;
Zheng-Tong DING
;
Jian-Jun WU
;
Yuping JIANG
;
- Publication Type:Journal Article
- Keywords:
Pigment epithelium,eye;
Dopamine;
Microcystis
- From:
Chinese Journal of Geriatrics
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the features of dopamine secretion of cultured bovine retinal pigmentary epithelial(RPE) cells. The level of dopamine and survival rate after passage and microencapsulation were also observed. MethodsThe primary culture of bovine RPE cells was made using enzyme digestion. After purification and identification the growth curve of the cell was observed. Alginate-polylysine-alginate(APA) microencapsulated cells were made with a high voltage electostatic system. The activity of the cells in the mirocapsule was investigated by trypan blue staining. The secretion of dopamine was determined by high performance liquid chromatography (HPLC) assay. ResultsThe cell had high purity of immunocytochemistry. The growth curve showed that the exponential growth occurred at the first 1~4 days. Dopamine content was first detected at the time point of 2 hour, and arrived at the peak at about 48 hour of the cultivation. The secretion of dopamine was not different between passages. Dopamine secretion was dramatically decreased in the first 4 days after microencapsulation (P
