Apoptosis and expression of Fas, TNF-? and Bcl-2 in patients’serum and cerebrospinal fluid with HAM/TSP
- VernacularTitle:人嗜T淋巴细胞病毒Ⅰ型相关性脊髓病/热带痉挛性截瘫患者血清和脑脊液中细胞凋亡及凋亡相关因子的表达
- Author:
Yingxiu XIAO
;
Xuan ZHENG
;
- Publication Type:Journal Article
- Keywords:
Paraparesis,tropical spastic;
Apoptosis;
Antigens,CD95;
Tumor necrosis factor;
Proto oncogence proteins c bcl 2
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis of peripheral blood lymphocyte cells in patients with HAM/TSP and the roles of Fas/FasL system, TNF ? and Bcl 2 protein in occurrence and progression of HAM/TSP Methods The single cell gel electrophoresis (comet assay) was used to detect the apoptosis of lymphocyte cells in patients with HAM/TSP The presence of sFas, sFasL in the serum and cerebrospinal fluid (CSF) of patients with HAM/TSP was evaluated by using an enzyme linked immunosorbent assay (ELISA) The Fas, TNF ? and Bcl 2 protein expressions were quanlitatively determined by flow cytometry from 8 cases of HAM/TSP, and compared with the controls and other inflammatory neurological diseases (OIND) Results (1) The apoptotic ratio of lymphocyte cells in patients with HAM/TSP (13 63%?0 48%) was significantly increased as compared to the controls (1 63%?0 57%, P 0 05) Levels of sFas and sFasL in CSF from patients with HAM/TSP ((0 19?0 07) ng/ml and (0 24?0 08) ng/ml) were both significantly increased than those from OIND ((0 11?0 06) ng/ml and (0 01?0 01) ng/ml, P 0 05) or patients with OIND (34 84?9 63, P
