Study on the expression of CD28 costimulatory molecule on human lymphocytes inhibited by special siRNA
- VernacularTitle:小干扰RNA特异性抑制淋巴细胞共刺激分子CD28基因表达的研究
- Author:
Ying ZHANG
;
Kailin XU
;
Xiuying PAN
;
Qunxian LU
;
- Publication Type:Journal Article
- Keywords:
RNA interference;
Small interfering RNA(siRNA);
Lymphocyte;
CD28 costimulate molecule
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of CD28 costimulatory molecule on human lymphocytes inhibited by siRNA.Methods:Three different siRNA (siRNA 1,siRNA 2,siRNA 3) were designed and synthysized and transfected into freshly isolated human lymphocytes with cationic liposome.At 24,48 and 72 h post transfection,the changes of CD28 expression were detected by flow cytometry,and the changes of CD28 mRNA levels were determined by semi quantitative RT PCR.Results:Different siRNA showed different reduction in CD28 expression.At 48 h post transfection,the degrees of reduction with siRNA 1,siRNA 2 and siRNA 3 were 22 10%?1 63%,73 50%?1 02% and 42 90%?0 89% respectively compared with the control ( P
