Studies of the pig interleukin 2(IL-2) eukaryon expression plasmid on cellular immune responses of BALB/c mice immuned with pcDNA-PRRSV-ORF5 DNA vaccine
- VernacularTitle:猪IL-2真核表达质粒对猪繁殖与呼吸综合征病毒ORF5基因疫苗诱导小鼠细胞免疫增强作用的研究
- Author:
Xiwen CHEN
;
Anchun CHENG
;
Mingshu WANG
;
Nigen XINI
;
Wenbo DOU
;
Pingying ZHANG
;
Xuemei LI
;
Gang WANG
;
Wumei LIU
- Publication Type:Journal Article
- Keywords:
Pig interleukin 2(IL-2) eukaryon expression plasmid;
Porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine;
Cellular immune response;
Mice
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of pig interleukin 2(IL 2) eukaryon expression plasmid on cellular immune responses of BALB/c mice immuned with pcDNA PRRSV ORF5 DNA vaccine.Methods:BALB/c mice were immunized with pcDNA PRRSV ORF5 DNA vaccine and pig interleukin 2(IL 2) eukaryon expression plasmid by the routes of co injection and DNA vaccine injection alone respectively, with PBS and pcDNA3 1(+) as controls. Fluoresecence Activated Cell Sorter(FACS),T lymphocyte proliferation test(MTT) were used to detect the number of CD4 +、CD8 + and the T lymphocyte proliferation in peripheral blood of mice vaccinated.Results:ConA response of T lymphocytes in blood was higher in experiment group than the control group ( P
