Studies on anti proliferation and inducing apoptosis effects of CIK cells against MGC 803 gastric cancer cell lines
- VernacularTitle:CIK细胞对MGC-803胃癌细胞株抗增殖及诱导凋亡作用的研究
- Author:
Shu SUN
;
Xuemei LI
;
Jianguang WANG
;
Wanshan YANG
;
Xiangdan LI
;
- Publication Type:Journal Article
- Keywords:
CIK cells;
MGC 803 gastric cancer cells;
Anti proliferation;
Apoptosis;
p53 protein expression;
p16 protein expression;
C myc protein expression
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the anti proliferation and inducing apoptosis effects of cytokine induced killer cells CIK cells on MGC 803 gastric cancer cell lines and to probe its underlying mechanism.Methods:To detect the anti proliferation and the cytotoxicity of CIK cells on MGC 803 gastric cancer line by MTT assay.The morphological changes of the apoptosis cell were observed by HE stain, scanning and transmission electron microscope. The positive expression of p53, p16,C myc were determined by immunocytochemistry (ICC).Results:MTT assay showed that the inhibitive rate inhanced obviously with the addition of Effect/Target rate and extension of time ( P
