Growth inhibiting effect on bladder carcinoma in vivo and localization of bispecific gene antibody(anti-human bladder carcinoma/anti-VEGF) by immune electron microscope
- VernacularTitle:抗人膀胱癌/抗血管内皮生长因子双功能基因抗体对膀胱癌的生长抑制作用
- Author:
Lucheng LIU
;
Haitao FAN
;
Hang GUO
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
Genes;
Antibody
- From:
Chinese Journal of Urology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immune localization of bispecific gene antibody (anti human bladder carcinoma/anti VEGF),and to determine whether bispecific gene antibody can inhibit growth of human experimental bladder carcinoma. Methods Immunohistochemistry and immune electron microscope were used to study the immune localization in 60 samples of bladder cancer (experiment group),and 20 samples from BPH cases served as control group.Bispecific antibody was injected into the sites in nude mice,which was adjacent to the xenograft tumor of human bladder transitional cell carcinoma.The tumor size was measured at different times.The microvascular density(MVD) and apoptotic index(AI) in the tumors were examined. Results Of the 60 bladder cancer samples 53 (88.3%) were positive for antigen,while only 1 (5.0%) of the 20 in control group was positive.Two weeks after treatment,the tumor size of experiment group was ( 21.47 ?6.57)mm 2,while that of control group was (59.85?15.43)mm 2.MVD of experiment group was 2148?109,while that of control group was 4056?367.AI of experiment group was 17.26,while that of control group was 7.09.The differences between the 2 groups were significant (each P
