Preparation of rmOP-1 and its protective effect on renal ischemia reperfusion injury
- VernacularTitle:重组鼠骨形成蛋白-1的制备及其对肾脏缺血-再灌注损伤的保护作用
- Author:
Yanjiang LI
;
Lijiang SUN
;
Zhenhua LU
;
- Publication Type:Journal Article
- Keywords:
Bone morphogenetic proteins;
Kidney;
Reperfusion injury;
Cytoprotection
- From:
Chinese Journal of Organ Transplantation
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of recombinant mouse OP 1 (rmOP 1) on renal ischemia reperfusion injury. Methods The full length open reading frame of mouse OP 1 was cloned by RT PCR. The rmOP 1 was prepared and its osteogenesis was assayed in vitro. Thirty Wistar rat models of renal ischemia reperfusion were randomly divided into 3 groups: preventive group, therapeutic group and control group. The former two groups were administered with rmOP 1 (250 ?g/kg) via the superior mesenteric vein at different time points. The renal function and structure were assessed by serum BUN, Cr, histological findings and the modified Miller's scoring. Results rmOP 1 showed osteoinductive activities in vitro. The level of serum BUN, Cr and Miller's scoring were higher in control group than the others ( P 0.05 ). Histological finding showed that rmOP 1 minimized the cell necrosis. Conclusion rmOP 1 has an protective effect on renal function and structure.
