The epitope analysis of HLA class I specific antibody in highly sensitized renal transplant recipients
- VernacularTitle:高致敏肾移植受者HLAI类抗体表位分析
- Author:
Xiaopeng YUAN
;
Wei GAO
;
Qifa YE
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Panel reactive antibody;
Antibody specificity;
HLA antigens
- From:
Chinese Journal of Organ Transplantation
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epitope of HLA class I specific antibody in the serum of highly sensitized patients (HSP). Methods The specificities and epitopes of HLA class I specific antibody from 54 HSP were monitored continually by enzyme linked immunosorbent assay (ELISA). Results 90.7 % of HSP had public epitopes of HLA class I antibody. The common public epitopes were 163E, 142 145TTKH, 83R, 71 74AQTD, 127K, 163R, 163L, 41T, 62 66RNTRN and 41 45ASPRT. The public epitopes were stable in the course of continual monitoring, and it could reflect antibody spectrum more accurately than antibody specificity analysis. Conclusion Most HSP produce HLA class I antibodies in a fairly predictable fashion. These antibodies direct against a small number of high frequency public amino acid residues with high immunogenicity. Analysis of public epitopes of HLA antibodies is very useful to predict acceptable mismatches.
