Expression of Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase in Autogenous Vein Grafts
- VernacularTitle:细胞外信号调节激酶和p38蛋白激酶在自体移植静脉中的表达
- Author:
Jun YANG
;
Xinhua HU
;
Qiang ZHANG
;
Zhiquan DUAN
;
- Publication Type:Journal Article
- Keywords:
Vein graft Mitogen-activated protein kinase Vascular remodeling
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of extracellular signal regulated kinase (ERK) and p38 mitogen activated protein kinase (p38 MAPK) in autogenous vein grafts during vascular remodeling.Methods An autogenous vein graft model was established by transplanting the right jugular vein to infrarenal abdominal aorta in 80 Wistar rats. Vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks and 8 weeks after surgery. Gene expression of ERK and p38 MAPK was measured by reverse transcription PCR. Western blot was used to detect the expression of protein products and phosphorylation protein products of ERK and p38 MAPK. Apoptosis of vascular smooth muscle cells (VSMCs) was determined by TUNEL. Proliferating cell nuclear antigen(PCNA) of VSMCs also was studied.Results The expression of ERK 1 mRNA and p38 MAPK mRNA increased considerably after surgery. ERK 1 mRNA reached the peak on the 7th day 〔(33.2?14.2)%, P
