Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2
- VernacularTitle:猪瘟病毒E2糖蛋白抗原表位的预测、多肽合成及特性研究
- Author:
Siguo LIU
;
Gang MA
;
Xinglong YU
;
Maolin ZHANG
;
Changchun TU
;
- Publication Type:Journal Article
- Keywords:
CSFV;
E2 protein;
Peptide synthesis
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Study on characteristics of two synthesizd peptides based on CSFV E2 protein. Methods:B cell epitopes of CSFV E2 antigen were predicted using accessibility and flexibility schemes, associated with antigenicity , secondary structure and multiple sites prediction. Two antigen peptides (Pep1 and Pep2) have been designed and synthesized and their reactivety were detected with 8 McAbs and antiserum against mE2 protein, then the peptides were conjugated with BSA and immunized rabbits respectively. Results:Both Pep1 and Pep2 could react with antiserum and McAb A11, Pep2 could interact with McAbD5 and McAbD8. Only Pep1 BSA conjugate can stimulate high level and specific antibodies.Conclusion: The peptide1 has good antigenicity.
