Effect and mechanism of fibroblast growth factor in promoting endothelia cell proliferation
- VernacularTitle:成纤维细胞生长因子促内皮细胞增生作用及其机制
- Author:
Wei-Li LV
;
Xue-Song XING
;
Yu-Lan DONG
;
Xiang-Hong YANG
- Publication Type:Journal Article
- Keywords:
Fibroblast growth factor;
Nuclear factor-kappa B;
ki-67antigen;
Proliferation
- From:
Chinese Journal of Trauma
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of fibroblast growth factor (FGF) on endothelia cell (EC) proliferation in vitro,TNP-470 and dexamethasone (Dex) and explore the possible mechanisms of FGF stimulating EC proliferation. Methods Cultured human umbilical vein endothelial cell (HUVEC) was divided into two groups: control group (Group DMEM) and experimental groups (Group FGF,Group Dex,Group TNP-470,Group FGF+Dex,Group FGF+TNP-470). The EC proliferation was quantified by a colorimetric assay using MTT reagent,the relative cell numbers at every time point of EC cycle analyzed with flow cytometer,and the expression of nuclear factor-kappa B p65 (NF-?B p65) and ki-67 detected with immunohistochemical method of SABC. Results FGF stimulated significantly EC proliferation,raised proliferation index (PI) and enhanced the expressions of NF-?B p65 and ki-67 in nuclear. TNP-470 reduced PI and TNP-470 and Dex suppressed EC proliferation and finally decreased the expressions of NF-?B p65 and ki-67 in nuclear. FGF could facilitate the inhibitory effect of TNP-470 and Dex. Conclusions The possible mechanism of EC proliferation stimulated by FGF is that FGF can activate NF-?B to promote the synthesis of DNA and EC mitosis. TNP-470 and Dex suppress proliferation and differentiation of EC but FGF may reverse such suppressive effect.
