Research on the Induction of Apoptosis in MCF-7 Cells Enhanced by Thapsigargin
- VernacularTitle:Thapsigargin对乳腺癌MCF-7细胞凋亡的诱导作用研究
- Author:
Jiangtao HAN
;
Zhenxiang YAO
;
- Publication Type:Journal Article
- Keywords:
Thapsigargin Breast carcinoma Apoptotic induction
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF 7. Methods Cells were treated with Thapsigargin and 5 FU in vitro . The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.Results Thapsigargin could increase the rates both of cell apoptosis and growth supression of MCF 7 cells induced by 5 FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF 7 cells considerably increased.Conclusion Thapsigargin apparently enhances the effect of apoptotic induction of 5 FU on MCF 7 cells, it is worthy of being further studied.
