Study on the potential and the effect of simvastatin on adipocytic differentiation of bone marrow stromal cells
- VernacularTitle:骨髓基质细胞成脂分化及辛伐他汀对其影响的实验研究
- Author:
Chunli SONG
;
Hongti JIA
;
Qingjun MA
;
Zhaoqing GUO
;
Gengting DANG
;
- Publication Type:Journal Article
- Keywords:
Simvastatin;
Bone marrow cells;
Osteoblasts;
Adipocytes;
Osteoporosis
- From:
Chinese Journal of Geriatrics
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the adipocytic differentiation potential of bone marrow stromal cells (BMS), and the effect of simvastatin on adipocytic differentiation of bone marrow stromal cells in vitro, and to elucidate the mechanisms of anabolic effect of simvastatin on bone formation. Methods BMS from femur and tibia of adult female BALB C mice were cultured in vitro. Changes of alkaline phosphatase (ALP) activity were determined after treatment with adipogenetic agonist (hydrocortisone 0 5 ?mol/L and indomethacin 60 ?mol/L, HI) for 6 days. Thenexpression of lipoprotein lipase (LPL) mRNA was detected by RT PCR after treatment with HI and different concentration of simvastatin for 72 h. Adipogenetic differentiation were also observed with Oil Red O staining and fluorescence activated cell sorting (FACS) after treatment with HI and different concentration of simvastatin or 100 ?g/L rhBMP 2 for 12 days. Results After BMS were treated with HI for 6 days, ALP activity was significantly decreased ( P
