Effect of ets-1 antisense oligoxydeonucleotide on invasive ability of gastric cancinoma cell line SGC-7901 in vitro
- VernacularTitle:ets-l反义寡核苷酸对胃癌细胞株SGC-7901体外侵袭力的影响
- Author:
Yinlu DING
;
Zhaoting LI
;
- Publication Type:Journal Article
- Keywords:
Gene,ets?Oligonucleotides,antisense?SGC 7901cells?Neoplasm invasiveness?In vitro
- From:
Chinese Journal of Current Advances in General Surgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ets 1 antisense oligoxydeonucleotide(ASODN)on invasive ability of gastric cancer cell line SGC 7901 in vitroMethods:Cultured cells were divided into three groups:control group,sense oligoxydeonucleotide(SODN)group and ASODN group.After transfection for 24h,cultured cells were harvested to detect the changes of ets lmRNA by the reverse transcription polymerase chain reaction method(RT PCR),growth inhibition was detected by colony forming unit assay,invasive ability was measured by the number of cells to penetrate polycarbonates coated with or without matrigel.Results:est 1 ASODN could underregulate the expression of ets 1mRNA,number of colony forming was significantly lower than the other two groups(24.2?4.8,47 6?8 1,44 3?7 6,P
