Expression and anti-hepatoma effect of TCR V?7 after being transfected into PBLs
- VernacularTitle:TCR V?7基因转染PBLs的表达及抗肝癌的作用
- Author:
Lanfeng XIAO
;
Jiahong YAO
;
Yuexia LIN
;
Al ET
- Publication Type:Journal Article
- Keywords:
TCR V?7 gene;
Transgene;
Laser Scanning Confocal Microscope;
Transmembrane protein;
Hepatoma cell apoptosis
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological effects of TCR on hepatoma cells by transfecting V?7 into lymphocytes.Methods:TCR V?7 gene was amplified by RT PCR and cloned to expression vector pLXSN. The recombinant was transferred into lymphocytes by Lipofectin Reagent transfection, then the lymphocytes were co cultured with hepatoma cells. The phenotype of lymphocytes was detected on the Flow Cytometry, the expression of TCR V?7.1 gene was detected by Laser Scanning Confocal Microscope ( LSCM) and the ultrastructure of the hepatoma cells was showed by electronic microscope.Results:The amount of the transmembrane protein expressed by TCR V?7.1 gene was increased significantly, and so was the amount of lymphocytes (P
