The preparation of anti-human bladder cancer/anti-VEGF bispecific antibody and in vitro study on cytotoxicity
- VernacularTitle:抗人膀胱癌抗VEGF双功能基因抗体制备及体外效应研究
- Author:
Lucheng LIU
;
Hang GUO
;
Haitao FAN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Bladder cancer;
VEGF;
Bispecific antibody
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyse and prepare bispecific antibody(BsAb, anti human bladder cancer/anti VEGF) which could enhance the effectiveness of tumor cell killing activity.Methods:Monoclonal antibodies against human bladder cancer and VEGF were used to prepare bispecific antibody.Results:IC50 of BsAb was 10 -9.5 ,while those of antibodies against human bladder cancer and VEGF were 10 -8.9 and 10 -8.3 .Conclusion:The effectiveness of BsAb was significantly higher than that of monoclonal antibodies. [
