The effect of receptor-associated protein in acetylcholine receptor of experimental autoimmune myasthenia gravis
- VernacularTitle:受体相关蛋白对实验性自身免疫性重症肌无力的乙酰胆碱受体的作用
- Author:
Xu ZHANG
;
Losen MARIO
;
MARC DE BAETS
;
- Publication Type:Journal Article
- Keywords:
Receptor associated protein;
Experimental autoimmune myasthenia gravis;
Acetylcholine receptor
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigation the effect of receptor associated protein (Rapsyn) in acetylcholine receptor (AChR) of end plate of experimental autoimmune myasthenia gravis (EAMG).Methods:Made and purified the pcDNA Rapsyn with genetic engineering technique and injected the muscle of vastus lateralis of left leg of Lewis rats, and then stimulated with electro square porator. EAMG induced by passive transfer mAb35 injected intraperitoneally, and monitor body weight and assessment clinical signs score. Sacrificed animal and removed the muscle of vastus lateralis of Lewis rats after 48 hour of induction of EAMG, measurement the AChR with radioimmunoassay (RIA) and calculated the AChR loss rate.Results:There were lower AChR loss rate found in muscle pretreated with pcDNA Rapsyn then muscle pretreated with Vector(as negative control).Conclusion:Rapsyn protein can protect the AChR of EAMG model. [
