Adoptive transfer of splenocytes of acute myocardial infarction rats Mediated myocardial injury
- VernacularTitle:过继转输急性心肌梗死大鼠脾细胞介导心肌损伤
- Author:
Rong TAO
;
Yuhua LIAO
;
Xiang CHENG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Acute myocardial infarction;
Autoimmune;
Ventricular remodeling
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the autoimmune response against self myocardial tissue in an experimental rats model of acute myocardial infarction (AMI) and reveal a potential role of autoimmune mediated myocardial injury involved in ventricular remodeling after AMI.Methods:An experimental animal model of AMI was adopted by in vivo ligation of left anterior descending branch (LAD) in Wistar rats After six weeks, spleens were removed and splenocytes were collected About 100?10 6～150?10 6 splenocytes were freshly transferred to syngeneic inbred rats Four weeks later, these recipient rats were anesthetized for hemodynamics analysis by catheter technique Antibody against cardiac myosin heavy chain (MHC) was screened by enzyme linked immunosorbent assay Histopathological studies were performed on all hearts Results:The antibody against cardiac MHC was positive in 8 of 22 AMI rats and recipient rats, and in 0 of 20 sham operation and recipient rats,P
