Antigenic epitopes analyses and expression of P-glycoprotein
- VernacularTitle:P-糖蛋白抗原表位分析及表达
- Author:
Xiangwei WANG
;
Lixin ZHANG
;
Tangjun YANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
multidrug resistance;
P glycoprotein;
antigenic epitopes
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study antigenic epitopes and expression of P glycoprotein.Methods:The secondary structure and surface properties of P glycoprotein was studied by many methods , designed a pair of primers to amplify DNA with PCR. The product was inserted into a pGEM T vector, sequenced and cloned into pGEX 2T vector, established a high expression recombinant vector named pGEX Pgp,which was transferred into DH5? and expressed , identified and purified. Results:Antigenic epitopes in extracellular fragment of P glycoprotein were identified in amino acid residues 82 115(I),741 760(IV) and 800 816(V). SDS PAGE analysis indicated that the expressed protein was about 30?10 3 (30 kD).Conclusions:High level expression of the target gene fragment was established for preparation of its antibody and biospanning its specific peptide using random phage library , which became the basis for the targeting treatment of MDR. [
