Role of p38MAPK in mediating TNF-?-induced apoptosis in rat glioma cells
- VernacularTitle:p38MAPK在介导TNF-?诱导大鼠胶质瘤细胞凋亡中的作用
- Author:
Bicheng ZHANG
;
Qing LI
;
Jing YE
;
Al ET
- Publication Type:Journal Article
- Keywords:
p38MAPK;
tumor necrosis factor;
glioma;
apoptosis;
SB202190
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the role of p38MAPK in mediating TNF ? induced apoptosis in rat glioma cells C6.Methods:The proliferation activity of C6 cells after the treatment by TNF ? was observed by MTT assay. The TNF ? induced apoptosis was detected by transmission electron microscopy and flow cytometry. The expression of p38MAPK was detected by SABC method and Westernblot. The effect of SB202190, a specific inhibitor of p38MAPK on TNF ? induced apoptosis was observed by flow cytometry and SABC method. Results:The inhibitory rate of TNF ? (2?10 5 U/L) on C6 cells was 43.75%. In the TNF ? treated group, apoptotic cells were observed by transmission electron microscopy and the apoptotic rate was 37.5% by flow cytometry, p38MAPK positive signals were found by SABC method and Westernblot. In the SB202190 treated group, the apoptotic rate was 7.0% and no p38MAPK signals were found.Conclusions:The apoptosis of C6 cells and expression of p38MAPK could be induced by TNF ?. The activation of p38MAPK promoted the apoptosis of C6 cells. [
