Chemosensitivity testing for human gastric cancer with short-term culture cells vs purified primary culture cells using the MTT assay
- VernacularTitle:人胃癌原代细胞短期培养与纯化培养体外药敏试验
- Author:
Hongwei XU
;
Chengyong QIN
;
Juren ZHU
;
Al ET
- Publication Type:Journal Article
- Keywords:
gastric cancer;
tumor cells culture;
MTT colorimetric assay;
chemosensitivity;
anticancer drug screening assay
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To evaluate the feasibility, advantages and disadvantages of chemosensitivity test of human gastric cancer using MTT assay with short term culture tumor cells contaminated by nonmalignant cells compared with purifiedly primary culture tumor cells.Methods:Fifty nine fresh samples from patients with gastric cancer were obtained from operating rooms. Chemosensitivity results were provided by the MTT assay with short term culture cells. The primary culture cells were purified by means of a series of methods such as repeated cell attachment, differential trypsinization and natural purification which removed fibroblasts and other nonmalignant cells. The same MTT assay was conducted using purified cells. Chemosensitivity results between short term method and purification method were compared for seven antitumor drugs. Results:The success rate of short term method using the MTT assay for chemosensitivity testing was 81 4% (48 of 59 patients),and for the purification method it was 50.8(30 of 59 patients). The average was 20.2?9.5 days when primary cells were cultured into purified cancer cells. The optical density ( A ) value correlated directly with the the number of tumor cells with the two methods( P
