The effect of allogeneic MHC Ⅰ gene modification on the immunologic character in bone marrow cells
- VernacularTitle:异基因MHCⅠ修饰对骨髓细胞的免疫学特性的影响
- Author:
Lanxiao WU
;
Kunyuan GUO
;
Yu QIN
;
Al ET
- Publication Type:Journal Article
- Keywords:
Mice;
MHC Ⅰ gene;
Retroviral expression vector;
Bone marrow cells
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of allogeneic MHC Ⅰ gene modification on the immunologic character in bone marrow cells and to investigate the mechanism of induce immunologic tolerance of MHC Ⅰ.Methods:The retroviral expression vector of BALB/C mice H 2D d gene was constructed and transducted into C57BL/6 mice bone marrow cells And the expression of H 2D d on the infected cells was detected by FACS technique Then observe the MLR between the BALB/C mice spleen T cells and the C57BL/6 mice bone marrow cells modified with H 2D d gene by MTT assay The cytotoxic activity of BALB/C mice NK cells to the C57BL/6 mice bone marrow cells modified with H 2D d gene was detected by LDH release assay Results:Either stimulation or response ability of the C57BL/6 mice bone marrow cells to the T cell from BALB/C mice spleen was significantly decreased after modified with H 2D d gene The cytotoxic activity of BALB/C mice NK cells to the C57BL/6 mice bone marrow cells modified with allogeneic MHC Ⅰ gene was significantly lower than which to the C57BL/6 mice bone marrow cells non modified Conclusion:It's maybe a way to induce immunologic tolerance in bone marrow transplantation by the means of modify the bone marrow cells with the MHC Ⅰ gene of the recipient
