Study of cytotoxicity in hTCRV?8.4 transfected human T lymphocytes to hepatocellular carcinoma cells
- VernacularTitle:TCRV?8.4基因转染人PBMC对肝癌细胞杀伤活性的研究
- Author:
Lanfeng XIAO
;
Licai HU
;
Yuexia LIN
;
Al ET
- Publication Type:Journal Article
- Keywords:
hTCRV ?8.4;
Gene cloning and transfection;
Lymphocytes;
Hepatocellular carcinoma cells BEL 7402
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immune state and the alteration of cytotoxicity to hepatocellular carcinoma cells BEL 7402 in hTCRV ? 8 4 gene transfeced PBMC after co culturing with HBV derived hepatocellular carcinoma cells BEL 7402. Methods:The hTCRV? 8 4 recombinant plasmid was transfered into peripheral blood mononuclear cells (PBMC).Flowcytometer analysis were used to assay the expression of TCRV?8.4 gene and the the immune state of hTCRV?8.4 gene modified PBMC.LDH release assay were used to test the cytotoxicity of the PBMC to hepatocellular carcinoma cells BEL 7402.Results:The expresstion of TCRV?8.4 in CD3 +T cell was increased significantly after gene transfection.The percentage of TCRV?8.4?CD122 + and CD19 +lymphocytes increased obviously after co cultured with BEL 7402,this indicates BEL 7402 stimulated the proliferation of these cells and cellular immunity.Transmission electronic microscope showed apoptosis in BEL 7402 induced by hTCRV?8.4 gene transferred PBMC.LDH release assay reveals increased cytotoxicity of hTCRV ?8.4 gene transfered PBMC to BEL 7402.Conclusion:The proliferation and the cytotoxicity were enhanced significantly in hTCRV ?8.4 modified PBMC after stimulated by HBV derived hepatocellular carcinoma cells BEL 7402.
