Production and identification of specific monoclonal antibodies against recombinant human interleukin-18(hIL-18)
- VernacularTitle:抗重组人白细胞介素18单克隆抗体的制备与特性研究
- Author:
Ying MA
;
Quanming ZOU
;
Yonghong ZHU
;
Al ET
- Publication Type:Journal Article
- Keywords:
Interleukin 18;
Monoclonal antibody;
Hybridoma
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To produce hybridoma cell lines of monoclonal antibodies against recombinant human interleukin 18.Methods:Hybridoma techniques were used to fuse BALB/C mice myeloma cell line SP2/0 with B lymphocytes of spleen of BALB/C mice which were immunostimulated with interleukin 18 antigen through polyethyleneglycol (PEG).Single cell lines which stably produce antibodies against interleukin 18 were picked out and identified by enzyme linked immunosorbent assay.Results:Two hybridoma cell lines were obtained and named 1C7 and 1F5,which produce monoclonal antibodies specificity against interleukin 18.They respectively own 88 and 92 chromosomes, the purities of IgG in ascites are all over 95% through affinity chromatography, the titer degrees of ascies are 1?10 -4 and 1?10 -5 respectively.Two antibodies are against two different antigen binding epitopes on the surface of interleukin 18.It was evaluated by Western blot that the two antibodies can bind to interleukin 18 specifically and are made of only single protein strap.Conclusion:Have prepared two hybridoma cell lines which stably secrete high titer and high specific monoclonal antibodies against interleukin 18,which provide the foundation for further studying the structure and the biological function of interleukin 18.
