Study of methods of PCR-ELISA in testing the RNA of platelet activating factor receptor
- VernacularTitle:酶联免疫法检测PAF受体RNA的聚合酶链反应产物
- Author:
Xinsheng YAO
;
Wanbang SUN
;
Hanwen JIANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
PCR;
Psoriasis;
ELISA
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the methods of PCR ELISA in testing the RNA of platelet activating factor receptor.Methods:Extracting the total RNA of 20 normal people and 20 psoriasis patients,the PAF R and ? actin RNA assayed by RT PCR,PAF R sense labelled by Biotin and antisense labelled by Digoxin,? actin sense labelled by Biotin and antisense labelled by fluorescein.The results of PCR were tested by ELISA and Agar gel electrophoresisa.Results:The positive number of PAF R RNA was 16 by Agar gel electrophoresis and 18 by PCR ELISA in 20 normal people;The positive number of PAF R RNA was 18 by Agar gel electrophoresisa and 20 by PCR ELISA in 20 Psoriasis patients;The positive number of ? actin was 20 in two groups with two methods.Conclusion:The sense and antisense labelled by different substance in PCR ELISA has high sensitivity and the course was simple in testing the PAF R RNA.
