Association between expression of P-selectin and interleukin 8 and tumor necrosis factor ? in trinitrobenzene sulphonic acid induced colitis in rats
- VernacularTitle:TNBS诱发大鼠结肠炎P选择素的表达及其与TNF-?、IL-8的相关性研究
- Author:
Hong HAN
;
Bing XIA
;
Junyan LIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
P selectin;
IL 8;
TNF ?;
Trinitrobenzene sulphonic acid;
Colitis;
Rats
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of P selectin in the colonic mucosa and on surfaces of platelets in circulation, and determine its association with IL 8 and TNF ? in trinitrobenzene sulphonic acid (TNBS) induced colitis in rat, in order to investigate the effects of P selectin on inflammation and thrombosis in inflammatory bowel disease Methods:TNBS was instilled into the colon of the rats to induce colitis Animals were sacrificed at the 24th hour, the 1st and the 2nd week after production Blood and colonic tissues were obtained P selectin on the platelets were detected by radioimmunoassay IL 8 in serum was detected by ELISA method Expression of P selectin and TNF ? in colonic mucosa were assessed with immunohistochemistry Results:P selectin in mucosa was increased in different time points compared with control group The positive expression was above 50% P selectin on the platelets wasn't increased at the 24th hour, but increased at the 1st and 2nd week (P
