Relationship Between Hepatocellular Apoptosis and Glycogen Contents During Hepatic Cold Preservation Reperfusion and Its Mechanism
- VernacularTitle:低温保存-再灌注肝脏肝细胞凋亡与肝细胞糖原含量的关系及bax基因在其中的作用
- Author:
Lijun TANG
;
Fuzhou TIAN
;
Yu WANG
;
Xiaomei GAO
- Publication Type:Journal Article
- Keywords:
Reperfusion injury Hepatocellular apoptosis Glycogen
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between hepatocellular apoptosis and glycogen contents during hepatic cold preservation reperfusion and its mechanism.Methods Based on the model of four groups of rabbit livers with different hepatocellular glycogen contents, hepatocellular apoptosis and bax gene expression were observed during hepatic cold preservation reperfusion.Results Apoptotic hepatocytes were obviously found in 60 minute reperfusing livers subsequent to 9 hour cold storage, and there was significant difference in the numbers of apoptotic hepatocytes among all the groups. In the same time, there was the close relationship between the levels of bax gene expression and the glycogen contents of hepatocytes.Conclusion Intracellular abundant glycogen may significantly depress the hepatocellular apoptosis during hepatic cold preservation reperfusion by decreasing hepatocellular bax gene expression.
