The Protective Effect of Hepatocyte Growth Factor on Grafted Structure after Small Bowel Transplantation in Rat
- VernacularTitle:肝细胞生长因子对大鼠移植小肠粘膜结构的保护作用
- Author:
Kezhou LI
;
Xiaoting WU
;
Ning LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor Transplantation Total parenteral nutrition Mucous structure
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of hepatocyte growth factor(HGF) on grafted mucous membrane of transplanted small bowel.Methods Total small bowel transplantation was made in inbred Wistar (RT1 k) rats heterotopically,either total parenteral nutrition (control group, n =10) or hepatocyte growth factor supplemented TPN (HGF group, n =10) was given to the recipients from the 2nd day to the 10th day postoperatively. Morphological parameters of the transplanted intestinal mucosa, such as mucosal villous height, villous width, crypt depth, mucosal thickness and villous surface area were observed. Variation of ultrastructure of transplanted epithelial cells was observed. Composition of mucosal protein and DNA content were tested. Results Comparison between HGF group and the control group were as follows. Mucosal villous height (298.79?22.31) ?m vs (176.45? 14.62) ?m, P =0.001, villous width (107.46? 12.34) ?m vs (74.20?16.85) ?m, P =0.004, crypt depth (104.56?11.17) ?m vs (74.45?8.34) ?m, P =0.000 5, mucosal thickness (409.53?35.83) ?m vs (259.38?24.65) ?m, P =0.003, and villous surface area (0.101? 0.011) mm 2 vs (0.041?0.005) mm 2, P =0.001 were found significantly increased in HGF group compared with control group, there were no obvious difference decrease as compared to pretransplant parameters.Mucosal protein composition was higher in HGF group than that in control group (89.65? 9.28) mg/g wet wt vs (53.73? 11.45) mg/g wet wt, P =0.012, baseline (92.64?10.52) mg/g wet wt, nearly equal to baseline; DNA composition was also high in HGF group (0.89?0.09) mg/g wet wt vs (0.51?0.06) mg/g wet wt, P =0.008, baseline (0.91? 0.09) mg/g wet wt. Nearly normal ultrastructure of the graft was maintained in HGF group, atrophied microvilli and broken mitochondrial crista were observed in control group.Conclusion Hepatocyte growth factor can improve mucosal structure, preserve enterocyte ultrastructure of isograft after small bowel transplantation in rat.
