Comparative study on gene expression difference between high and low metastatic human hepatocellular carcinoma cell strains
- VernacularTitle:肝癌高低转移细胞株差异表达基因的初步研究
- Author:
Xin WU
;
Huliang JIA
;
Yinkun LIU
;
Al ET
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
hepatocellular carcinoma;
metastasis;
cDNA microarray;
chromosome 8p;
cultured tumor cells
- From:
China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the difference in gene expression profile between HCC cell strains with high and low metastatic potential.Methods:The gene expression profile of high and low metastatic HCC cell strains were analyzed by cDNA microarray, and genes with differential expression were further verified by RT PCR.Results:384 differentially expressed genes were detected between high and low metastatic HCC strains, making up 32% of the total genes studied. RT PCR analysis on 4 genes chosen demonstrated the same results as those revealed by the array technique.Conclusions:Obvious difference in gene expression patterns was observed between high and low metastatic HCC cell strains, chromosome 8p may contain metastasis promote and inhibitory genes.
