Inhibition of activation of NF-?B enhanced apoptosis induced by cytosine arabinoside in leukemic cells
- VernacularTitle:阿糖胞苷诱导NF-?B活化与白血病细胞凋亡的关系
- Author:
Xiaoping XU
;
Jianhui SHI
;
Zongliang ZHANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
cytosine arabinoside;
NF ?B;
apoptosis;
cultured tumor cells
- From:
China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To explore effects of dexamethasone and vincristine on apoptosis and activation of NF ?B of HL60 n cells induced by cytosine arabinoside (Ara C). Methods:Apoptosis induced by Ara C in HL60 n cells was analysed by Tunel and DNA electrophoresis. The DNA binding activation of NF ?B of HL60 n cells was determined by electrophoretic mobility shift assay(EMSA).Results:The apoptosis and activation of NF ?B of HL60 n cells could be induced by Ara C. Dexamethasone 1?mol/L and vincristine 0.1?mol/L increased significantly the apoptosis induced by Ara C (increased by 39.1% and 59.2%, P
