The relationships between structure and function of human transmembrane tumor necrosis factor-?
- VernacularTitle:跨膜型TNF-?细胞毒作用与其功能域的关系
- Author:
Fang ZHENG
;
Zhuoya LI
;
Feili GONG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Transmembrane TNF ?;
Receptor;
Site directed mutation;
Cytotoxicity;
Function region
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the region of human transmembrane tumor necrosis factor alpha (TM TNF?),essential for cytotoxic activity against mouse L929 cell.Methods:Single amino acid substituted TM TNF? mutant proteins(muteins) were produced by in vitro transcription linked translation techniques.An expression cDNA for TM TNF? was site directed mutagenized by recombinant PCR.7 single amino acid substituted TM TNF? muteins were generated and assayed for cytotoxic activity.Results:The cytotoxic activities of TM TNF? muteins,eg,TM TNF? -71/Lys was
